<html><head></head><body><div class="yahoo-style-wrap" style="font-family:times new roman, new york, times, serif;font-size:16px;"><div dir="ltr" data-setdir="false">Dear all,</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">After performing the structure comparison of 2 structures using the "MatchMaker" tool of the chimera software,</div><div dir="ltr" data-setdir="false">I need to run an alignment of the structure sequences using the "align chain sequences" tool of the chimera.</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Unfortunately, I am getting the following error message in the reply log that persists for weeks:</div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false"><div><div>Traceback from web service request (connection setup):</div><div>Traceback (most recent call last):</div><div> File "C:\Program Files\Chimera 1.12\share\WebServices\opal_client.py", line 11, in __init__</div><div> self._setup(serviceName, opalURL, sessionData)</div><div> File "C:\Program Files\Chimera 1.12\share\WebServices\opal_client.py", line 50, in _setup</div><div> self.sudsClient = Client(self.serviceURL + "?wsdl", **kw)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\client.py", line 115, in __init__</div><div> self.wsdl = reader.open(url)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\reader.py", line 150, in open</div><div> d = self.fn(url, self.options)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\wsdl.py", line 136, in __init__</div><div> d = reader.open(url)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\reader.py", line 74, in open</div><div> d = self.download(url)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\reader.py", line 92, in download</div><div> fp = self.options.transport.open(Request(url))</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\transport\https.py", line 62, in open</div><div> return HttpTransport.open(self, request)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\transport\http.py", line 67, in open</div><div> return self.u2open(u2request)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\site-packages\suds_jurko-0.6-py2.7.egg\suds\transport\http.py", line 132, in u2open</div><div> return url.open(u2request, timeout=tm)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 437, in open</div><div> response = meth(req, response)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 550, in http_response</div><div> 'http', request, response, code, msg, hdrs)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 469, in error</div><div> result = self._call_chain(*args)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 409, in _call_chain</div><div> result = func(*args)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 656, in http_error_302</div><div> return self.parent.open(new, timeout=req.timeout)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 431, in open</div><div> response = self._open(req, data)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 449, in _open</div><div> '_open', req)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 409, in _call_chain</div><div> result = func(*args)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 1240, in https_open</div><div> context=self._context)</div><div> File "C:\Program Files\Chimera 1.12\bin\lib\urllib2.py", line 1197, in do_open</div><div> raise URLError(err)</div><div>URLError: <urlopen error [SSL: CERTIFICATE_VERIFY_FAILED] certificate verify failed (_ssl.c:590)></div><div><br></div><div>Typically, if you get a TypeError, it's a problem on the remote server</div><div>and it should be fixed shortly. If you get a different error or</div><div>get TypeError consistently for more than a day, please report the</div><div>problem using the Report a Bug... entry in the Help menu. Please</div><div>include the traceback printed above as part of the problem description.</div><div>Traceback from web application request:</div><div>Traceback (most recent call last):</div><div> File "C:\Program Files\Chimera 1.12\share\WebServices\appWebService.py", line 48, in _initApp</div><div> self.backend = Backend(service, url)</div><div> File "C:\Program Files\Chimera 1.12\share\WebServices\opal_client.py", line 21, in __init__</div><div> raise NonChimeraError("Web service appears "</div><div>NonChimeraError: Web service appears to be down. See Reply Log for more details.</div><div>Service 'opal:ClustalOmegaService' is unavailable. See Reply Log for more details.</div><div><br></div><div dir="ltr" data-setdir="false">Can you please help?</div></div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false"><br></div><div dir="ltr" data-setdir="false">Thanks a lot for your time,</div><div dir="ltr" data-setdir="false">Chara</div></div></div></body></html>