<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Hi Antonio,<div class=""><br class=""></div><div class="">The error "<i class="">ImportError: No module named replyobj" </i><span class="" style="font-style: normal;">means Chimera cannot find its message logging module called "replyobj". That suggests that your Chimera installation is broken, and does not appear to be a problem specifically with BLAST. I would suggest installing the current Chimera version 1.16.</span><div class=""><br class=""></div><div class="">If that does not fix it, use the Report a Bug... button that should appear when this error message is shown. Or if that button does not appear use Chimera menu entry Help / Report a Bug....<br class=""><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On May 31, 2022, at 8:05 AM, Antonio Díaz Quintana via Chimera-users <<a href="mailto:chimera-users@cgl.ucsf.edu" class="">chimera-users@cgl.ucsf.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
<meta http-equiv="content-type" content="text/html; charset=UTF-8" class="">
<div class=""><p class="">Dear all, <br class="">
</p><p class="">Recently, I stopped being able to perform a BLAST search from the
SEQUENCE tool in UCSF Chimera. Is there anything I could do to fix
this?<br class="">
</p><p class="">Log message follows:</p><p class=""><i class="">Web Service: BlastProtein2 is a Python wrapper that calls
blastp to search nr or pdb for sequences similar to the given
protein sequence</i><i class=""><br class="">
</i><i class="">Opal service URL:
<a class="moz-txt-link-freetext" href="http://webservices.rbvi.ucsf.edu/opal2/services/BlastProtein2Service">http://webservices.rbvi.ucsf.edu/opal2/services/BlastProtein2Service</a></i><i class=""><br class="">
</i><i class="">Opal job URL:
<a class="moz-txt-link-freetext" href="http://webservices.rbvi.ucsf.edu/appBlastProtein2Service16540092552761301435050">http://webservices.rbvi.ucsf.edu/appBlastProtein2Service16540092552761301435050</a></i><i class=""><br class="">
</i><i class="">Traceback (most recent call last):</i><i class=""><br class="">
</i><i class=""> File "C:\Program Files\Chimera
1.15rc\share\chimera\tasks.py", line 179, in runStatusCB</i><i class=""><br class="">
</i><i class=""> task.statusCBList[0]()</i><i class=""><br class="">
</i><i class=""> File "C:\Program Files\Chimera
1.15rc\share\blastpdb\ParserBlastP.py", line 434, in _statusCB</i><i class=""><br class="">
</i><i class=""> self.finishCB(self.params, output)</i><i class=""><br class="">
</i><i class=""> File "C:\Program Files\Chimera
1.15rc\share\blastpdb\gui.py", line 424, in _finish</i><i class=""><br class="">
</i><i class=""> self.fetchColumnValues()</i><i class=""><br class="">
</i><i class=""> File "C:\Program Files\Chimera
1.15rc\share\blastpdb\gui.py", line 449, in fetchColumnValues</i><i class=""><br class="">
</i><i class=""> info = fetch_pdb_info(pdb_ids)</i><i class=""><br class="">
</i><i class=""> File "C:\Program Files\Chimera
1.15rc\share\blastpdb\pdbinfo.py", line 151, in fetch_pdb_info</i><i class=""><br class="">
</i><i class=""> import replyobj</i><i class=""><br class="">
</i><i class="">ImportError: No module named replyobj</i><br class="">
</p><p class="">Best,</p><p class="">Antonio<br class="">
</p>
<pre class="moz-signature" cols="72">--
Dr. Antonio J. Díaz Quintana
Instituto de Investigaciones Químicas
Universidad de Sevilla - CSIC
Avda. Americo Vespucio 49
41092 Sevilla
Spain
Phone: +34 954 48 9631</pre>
</div>
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