<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="">Hi Milad,</div><div class=""><br class=""></div>An easy way to make a fusion protein is to use ChimeraX and run AlphaFold to predict the structure. AlphaFold is not available in our old Chimera program -- no new features have gone into Chimera in over 5 years and many new capabilities are in ChimeraX.<div class=""><br class=""></div><div class="">In ChimeraX, use menu Tools / Structure Prediction / AlphaFold, paste in your fusion protein sequence and press Predict. You may need to log into your Google account since the calculation is run on Google Colab servers. Calculation time is 5 minutes to a few hours depending on sequence length.</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Aug 4, 2022, at 7:40 AM, Eric Pettersen via Chimera-users <<a href="mailto:chimera-users@cgl.ucsf.edu" class="">chimera-users@cgl.ucsf.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><meta http-equiv="Content-Type" content="text/html; charset=us-ascii" class=""><div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><br class=""><div class=""><br class=""><blockquote type="cite" class=""><div class="">Begin forwarded message:</div><br class="Apple-interchange-newline"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;" class=""><span style="font-family: -webkit-system-font, "Helvetica Neue", Helvetica, sans-serif;" class=""><b class="">From: </b></span><span style="font-family: -webkit-system-font, Helvetica Neue, Helvetica, sans-serif;" class="">Milad Mapar <<a href="mailto:milad.mapar@yahoo.com" class="">milad.mapar@yahoo.com</a>><br class=""></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;" class=""><span style="font-family: -webkit-system-font, "Helvetica Neue", Helvetica, sans-serif;" class=""><b class="">Subject: </b></span><span style="font-family: -webkit-system-font, Helvetica Neue, Helvetica, sans-serif;" class=""><b class="">Making Fusion Proteins using Chimera</b><br class=""></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;" class=""><span style="font-family: -webkit-system-font, "Helvetica Neue", Helvetica, sans-serif;" class=""><b class="">Date: </b></span><span style="font-family: -webkit-system-font, Helvetica Neue, Helvetica, sans-serif;" class="">August 4, 2022 at 6:26:46 AM PDT<br class=""></span></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;" class=""><span style="font-family: -webkit-system-font, "Helvetica Neue", Helvetica, sans-serif;" class=""><b class="">To: </b></span><span style="font-family: -webkit-system-font, Helvetica Neue, Helvetica, sans-serif;" class="">"<a href="mailto:pett@cgl.ucsf.edu" class="">pett@cgl.ucsf.edu</a>" <<a href="mailto:pett@cgl.ucsf.edu" class="">pett@cgl.ucsf.edu</a>><br class=""></span></div><br class=""><div class=""><div class=""><div class="yahoo-style-wrap" style="font-family:Helvetica Neue, Helvetica, Arial, sans-serif;font-size:16px;"><div dir="ltr" data-setdir="false" class="">Dear Eric</div><div dir="ltr" data-setdir="false" class=""><br class=""></div><div dir="ltr" data-setdir="false" class="">Hello</div><div dir="ltr" data-setdir="false" class=""><br class=""></div><div dir="ltr" data-setdir="false" class="">With thanks to your support on Chimera, could you please help me to make a Fusion Protein using Chimera 1.16</div><div dir="ltr" data-setdir="false" class=""><br class=""></div><div dir="ltr" data-setdir="false" class="">Any links to tutorials or hints would be appreciated </div><div dir="ltr" data-setdir="false" class=""><br class=""></div><div dir="ltr" data-setdir="false" class="">Many thanks </div><div dir="ltr" data-setdir="false" class=""><br class=""></div><div dir="ltr" data-setdir="false" class="">Pharmaceutical Biotechnology PhD Student</div><div dir="ltr" data-setdir="false" class="">Milad </div></div></div></div></blockquote></div><br class=""></div>_______________________________________________<br class="">Chimera-users mailing list: <a href="mailto:Chimera-users@cgl.ucsf.edu" class="">Chimera-users@cgl.ucsf.edu</a><br class="">Manage subscription: <a href="https://www.rbvi.ucsf.edu/mailman/listinfo/chimera-users" class="">https://www.rbvi.ucsf.edu/mailman/listinfo/chimera-users</a><br class=""></div></blockquote></div><br class=""></div></body></html>