<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="">To run AlphaFold for more than one sequence you need the ChimeraX daily build (aka 1.4). The error you got suggests you are using ChimeraX 1.3 which only handles a single sequence.</div><div class=""><br class=""></div>The Internal Server Error can be ignored -- it may be the UCSF servers are down, it is just trying to check what plugins are available.<br class=""><div><br class=""></div><div><span class="Apple-tab-span" style="white-space:pre"> </span>Tom</div><div><br class=""><blockquote type="cite" class=""><div class="">On Mar 23, 2022, at 6:29 PM, Gawargi, Flobater I via ChimeraX-users <<a href="mailto:chimerax-users@cgl.ucsf.edu" class="">chimerax-users@cgl.ucsf.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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<div class="">Hi, </div>
<div class="">I am a new Chimerax user and I have encountered a problem that I hope you can help me fix. I installed 1.4 version and it shows "Startup Messages notes available bundle cache has not been initialized yet Updating list of available bundles failed:
Internal Server Error” all I am trying to do is use alpha fold to predict structure of 2 proteins separated by comma. And it shows this error "Missing or invalid "sequences" argument: Sequences argument "cvfpfvflgkqyssctsdgrrdgrlwcattsnfdtdkkwgfcpdqgyslflvaahefghalgldhssvpealmyplysylegfplnkddidgiqylygrgskpdprppattttepqptapptmcptipptayptvgptvgptgapspgptsspspgptgapspgptapptagsseasteslspadnpcnvdvfdaiaeiqgalhffkdgwywkflnhrgsplqgpfltartw"
is not a chain specifier, alignment id, UniProt id, or sequence characters” </div>
<div class="">I am using an M1 MacBook, but I have also tried it on windows running windows 10 and got the same issue. I would appreciate your input to fix this issue. </div>
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<div class="">Thank you, </div>
<div class="">Flobater Gawargi </div>
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