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<p class="MsoNormal">Hello </p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have recently tried to use the bond rotation feature of Chimera X (Windows 10), and I have not been able to get it to work.</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have tried the Bond rotation button on the toolbar, and the torsion command to no avail.</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The bond rotation button does not seem to do anything even when I click and drag with right click.</p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The Torsion command states that my bond<i> </i><i><span style="color:black">“</span></i><i><span style="color:black">is part of a ring/cycle and cannot be rotated” or “</span></i><span style="color:black">To set torsion, middle two atoms
(/A ARG 543 N C) must be bonded;they aren't”<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:13.5pt;font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">I want to ask how do I use this function to rotate upon a simple amide bond.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">I am not sure am I just selecting the wrong atoms/bonds or is the function just locked for this PDB.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">The PDB file I am using is 7Y4P and I am trying to rotate about residues 543-544.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">I have also tried to do it in normal Chimera’s rotation command with no results.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">Thank you any advice would be welcome.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif;color:black">Chun Po Fung<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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