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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">Hi Elaine,
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><br>
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">I am uploading to the EMDB (wwPDB Deposition) and I am doing a composite deposition.
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><br>
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">I saw there are TER XXXX after each chain but lack in the Chain X.
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><br>
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">Thanks,
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<div class="elementToProof"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">Hong<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Elaine Meng <meng@cgl.ucsf.edu><br>
<b>Sent:</b> Wednesday, December 21, 2022 10:27 AM<br>
<b>To:</b> HONG ZHAN <hzhan3@wisc.edu><br>
<b>Cc:</b> ChimeraX Users Help <chimerax-users@cgl.ucsf.edu><br>
<b>Subject:</b> Re: [chimerax-users] PDB upload TER card error</font>
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<div class="PlainText">Hi Hong,<br>
Uploaded where? We try to write correct PDB format, but some websites that use uploaded PDBs may be pickier than others.<br>
<br>
Anyway, the solution is exactly what the error message says:<br>
Just use your favorite text-editor to add a line with first 3 characters TER after the X chain coordinates (ATOM lines).<br>
<br>
Intro to PDB format:<br>
<<a href="https://rbvi.ucsf.edu/chimerax/docs/user/formats/pdbintro.html">https://rbvi.ucsf.edu/chimerax/docs/user/formats/pdbintro.html</a>><br>
<br>
I hope this helps,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D. <br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
<br>
> On Dec 21, 2022, at 6:56 AM, HONG ZHAN via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:<br>
> <br>
> Dear all, <br>
> <br>
> I saved my protein coordinates in ChimeraX as pdb (it has multiple chains), however, when I was doing upload on the PDB, it shows this error, I am using ChimeraX 1.5rc202211091945 on Linux ubuntu 22.04 LTS.
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> <br>
> Coordinates issues: <br>
> <br>
> ERROR: There is no TER card at the end of polymer chain 'X'. All polymer chains should have a TER card at the end of each chain. Please add TER card and restart again.<br>
> <br>
> Many thanks!<br>
> Hong<br>
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