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Dear Elaine,</div>
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Thank you so much for your fast response.</div>
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I just checked the documentation and I'm a little uncertain if I was understanding it correctly.</div>
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May I ask if I want to predict a protein complex, all I need to do is to put a comma between the 2 sequence and if there is an interaction, the complex will be automatically predicted and there is nothing more I need to select?</div>
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Again, thank you so much for your help.</div>
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Best Wishes<br>
Yiling Lan</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Elaine Meng <meng@cgl.ucsf.edu><br>
<b>Sent:</b> Thursday, April 20, 2023 12:42 PM<br>
<b>To:</b> Yiling Lan <ylan03@syr.edu><br>
<b>Cc:</b> chimerax-users@cgl.ucsf.edu <chimerax-users@cgl.ucsf.edu><br>
<b>Subject:</b> Re: [chimerax-users] Enquires about predicting protein interaction with chimeraX/AlphaFold</font>
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<div class="PlainText">Dear Yiling Lan,<br>
The video is older. In the newer versions of ChimeraX, the AlphaFold tool does not have this option (it was removed), so you don't have to worry about it. I would recommend using the newer versions of ChimeraX because there is a continuous improvement process.
Also, the older versions may not work any more because AlphaFold or ColabFold itself has also changed during this time.<br>
<br>
The current (new) documentation for the AlphaFold tool is here:<br>
<<a href="https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html">https://rbvi.ucsf.edu/chimerax/docs/user/tools/alphafold.html</a>><br>
<br>
I hope this helps,<br>
Elaine<br>
-----<br>
Elaine C. Meng, Ph.D.<br>
UCSF Chimera(X) team<br>
Department of Pharmaceutical Chemistry<br>
University of California, San Francisco<br>
<br>
> On Apr 20, 2023, at 10:11 AM, Yiling Lan via ChimeraX-users <chimerax-users@cgl.ucsf.edu> wrote:<br>
> <br>
> Dear ChimeraX Developers,<br>
> <br>
> Hope this email finds you well.<br>
> I'm currently trying to predict the interaction of 2 proteins after watching the videos on youtube (Running AlphaFold to Predict Protein Complexes from ChimeraX). after installing the CimeraX (UCSF ChimeraX version: 1.6rc202304140213 (2023-04-14) version)
but I did not see the check box for "Is prokaryotes? used for predicting complex).
<br>
> May I ask if in newer version the check box for complex prediction is at somewhere else? If it does not bother too much, could you please let me know if there is anyway I can run complex prediction on 1.6rc?<br>
> Thank you so much.<br>
> Best Wishes<br>
> Yiling Lan<br>
> <Screenshot 2023-04-20 at 1.05.48 PM.png>_______________________________________________<br>
> ChimeraX-users mailing list<br>
> ChimeraX-users@cgl.ucsf.edu<br>
> Manage subscription:<br>
> <a href="https://www.rbvi.ucsf.edu/mailman/listinfo/chimerax-users">https://www.rbvi.ucsf.edu/mailman/listinfo/chimerax-users</a><br>
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