[Chimera-users] visualizing a confocal image stack

[William Beaver]

Hello.  I have a stack of intensity images (8bit) taken from confocal  
microscopy of dapi expression in Drosophila that I'd like to  
visualize.  I cannot figure out the format that the volume viewer  
requires to view the data; more specifically, none of the formats in  
'registered file types' are familiar to me and I've been unable to  
find anything about them to sort out which format I should convert  
to.   I have read the image sequences into matlab (3d matrix) and can  
write output to just about anything from there if I know the format.

Does anyone have an idea for what the best/easiest/only format I  
should use? A pointer to a spec on the format if it's not something  
trivial (like matlab 'save stack.txt varname -ascii') would be  
appreciated.

If it makes a difference, at some point soon I'd like to visualize  
more channels that just dapi (dll, for example) simultaneously.

Thanks in advance.   I really do appreciate it!
-William

------------
William Beaver
wbeaver at cs.ucsd.edu



-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://plato.cgl.ucsf.edu/pipermail/chimera-users/attachments/20060919/49309792/attachment.html>