[Chimera-users] creating a peptide bond between two fragments

[Glenn Millhauser]

Hi Folks,
   I am trying to add a four amino acid segment to the N-terminus of a protein in the pdb.  I can generate the four residue stretch and move its C-term close to the N-term of the structured protein.  I can then select the C' and N atoms, which are about 1.0 Angstrom apart.  At that point, I am stuck.  Not sure how to add a bond and then create a proper structure.  I've played around with Join Models, but no luck.  Without a peptide bond recognized by Chimera, ribbons are discontinuous.  Any ideas?
Many thanks,
glenn


Glenn L. Millhauser
Department of Chemistry & Biochemistry
UC Santa Cruz
Santa Cruz, CA 95064
831 459 2176 voice
831 566 3337 cell
831 459 2935 fax
831 824 4645 google voice

glennm at ucsc.edu

http://chemistry.ucsc.edu/~glennm
http://www.chemistry.ucsc.edu/faculty/millhauser.html










-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://plato.cgl.ucsf.edu/pipermail/chimera-users/attachments/20110607/860026c0/attachment.html>