[Chimera-users] Fitting multiple atomic structures in a density map

[Anindito Sen]

Dear All,

I need to fit atomic structures of two different proteins of different sizes in a density map. The location of both the proteins in the map is well known. While using the fitting tool, I can get the atomic structure of the bigger protein docked very well, the problem arises while fitting  the smaller one. The fit option fails and docks the structure into the region of the density map where the atomic structure of the larger protein is docked , an incorrect position. 

How can I fit both the atomic structures in the density map correctly? The resolution of the density map is ~10Å  at FSC cutoff of 0.5

Thanks

Andy
 
Dr. Anindito Sen (Ph.D)
>Department of Cell Biology & Anatomy
>Graduate School of Medicine
>University of Tokyo
>Tel & fax: +81-3-5841-3339
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