[Chimera-users] how to build missing loops

[Elaine Meng]

Dear Haiping Zhang,
I see, your PDB file does not have SEQRES.  My previous instructions assumed you had a structure from the PDB database that was missing coordinates for residues that were in SEQRES.

In your case, PDB file without SEQRES, you need to make a sequence file that has the GGGGGG included in the sequence where you want it, in plain text FASTA format:
<http://www.ncbi.nlm.nih.gov/BLAST/fasta.shtml>

Then open both the structure PDB file (yourfile.pdb) and the sequence FASTA file (yourfile.fasta) in Chimera.  Make sure the sequence is associated with the structure (sequence window menu: Structure… Associations), 
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/multalignviewer/multalignviewer.html#association>

and then proceed to use the Modeller loop-building interface as in my previous message.

If you never used Modeller with Chimera before, you have to get a Modeller license key to enter into the interface (it is free for noncommercial use, you just have to go to the Modeller website and sign up, they will email it to you).

I hope this helps,
Elaine
----------
Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco



On Sep 26, 2013, at 6:29 PM, 张海平 <spr8249618 at 163.com> wrote:

> Dear professor Elaine Meng：
>    I can not add a GGGGGGG sequence to the place between 182 and 183 residues by the Modeller interface for building loops. My structure is in a attachment file. Thanks for your help. 
> best wishes
> Haiping zhang
>