[Chimera-users] Adding sugars to protein at specific amino acid glycosylation site

[T T]

Dear Mr/Ms,
 Good morning. My name is Tommy from San Diego Biomedical Research
Institute. I am currently learning to use the chimera software. Great work
in creating a program for protein and DNA visualization.

I have one question to ask regarding the subject above. Is there a way to
manually add sugar component onto an existing pdb file?

To be precise, I am currently working with HIV-1 envelope. I have the pdb
files that features the structure of HIV-1 trimer Env. I wanted to add
glycan at specific sites to show steric hindrance to antibody binding.

Another question.
Is there a way to superimpose 2 pdb files into one image? For example, I
have one pdb file for HIV-1 Env and another pdb file for antibody. Can I
"merge" these two pdb files as one image?

Also, assuming that it is possible to "merge" two different pdb into one
image, is there a way to rotate one protein without affect the orientation
of the other protein?

Any advice/tip is greatly appreciated.
Thank you.

Sincerely,
Tommy
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