[Chimera-users] Difference map

[Elaine Meng]

Hi Yangqi,
Yes, see command “vop subtract”:

<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#subtract>

Keep in mind that if the two aren’t on the same grid, there may be small differences due to the need to sample them at the same points to do the subtractions.
I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Nov 16, 2018, at 4:39 PM, Yangqi Gu <yangqi.gu at yale.edu> wrote:
> 
> Dear Chimera users,
> I am trying to examine two cryo-EM maps and want to know if there is a subtle change between these two. I want to calculate the difference map, is it doable in Chimera?
> Best,
> Yangqi