[Chimera-users] Help with symmetry of fusion protein

[Hasan khan]

Hi there I was hoping I could get some help with a modelling challenge that
I am having as I am very new to protein modelling as a whole and Chimera.

So I developed a fusion protein of a single monomer of an encapsulin
structure (PDB:3DKT), I wanted to build the final icosahedral structure
with the added fusion protein. So I tried to align 10 of the fusion
monomers to the di-pentamer of the original PDB file and ran the sym
command generating the native structure with the added fusion protein.
I am unable to save this symmetry structure and I'm not sure if its due to
the alignment of the 10 monomers onto the di-pentamer that I did first and
then ran the sym command using coordinates of the original PDB or if
Chimera is not able to produce coordinates for the structure in a new PDB.
When I export it as a PDB the coordinates are not saved. So I was wondering
if there is a way I could save this newly generated structure as other
methods are not currently working.

Thank you
Kind regards
HK
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://plato.cgl.ucsf.edu/pipermail/chimera-users/attachments/20200414/14930748/attachment.html>