[chimerax-users] Labeling canine Apolipoprotein A-I

[Moradkhan, Tiglath A]

Hi

I'm trying to model the N-terminus of mature canine Apolipoprotein A-I using Chimera. However the actual sequence in uniprot is not that of the mature protein. Here is the sequence:


MKAALLTLAVLFLTGSQARHFWQQDEPQSPWDRVKDLATVYVDAVKDSGRDYVAQFEASA
LGKQLNLKLLDNWDSLSSTVTKLREQIGPVTQEFWDNLEKETEVLRQEMSKDLEEVKQKV
QPYLDDFQKKWQEEVELYRQKVAPLGSELREGARQKLQELQEKLSPLAEELRDRARTHVD
ALRAQLAPYSDDLRERLAARLEALKEGGGASLAEYHARASEQLSALGEKARPALEDLRQG
LLPVLESFKVSLLAAIDEATKKLNAQ


The sequence of the mature form is:

DEPQSPWDRVKDLATVYVDAVKDSGRDYVAQFEASA
LGKQLNLKLLDNWDSLSSTVTKLREQIGPVTQEFWDNLEKETEVLRQEMSKDLEEVKQKV
QPYLDDFQKKWQEEVELYRQKVAPLGSELREGARQKLQELQEKLSPLAEELRDRARTHVD
ALRAQLAPYSDDLRERLAARLEALKEGGGASLAEYHARASEQLSALGEKARPALEDLRQG
LLPVLESFKVSLLAAIDEATKKLNAQ


According to how the sequence is labeled in uniprot the first aspartate residue of mature canine

Apolipoprotein A-I starts at position 25, and this what Chimera uses. However, for the mature

protein this should start at position 1. How can I change the numbering system so the first

aspartate residue starts at position 1?


I would be very glad for your advice.


Thank you.

Tiglath

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