[chimerax-users] Troubles when saving a map

[Vadim Kotov]

Hi, Stéphane,

  The pixel size is just a value in the header of the MRC file, and you 
can change it with Relion:

relion_image_handler --i input.mrc --o output.mrc --force_header_angpix 
[new_value]

Best wishes,
Vadim Kotov
Postdoctoral fellow, Löw group
EMBL Hamburg


On 17.06.21 10:07, Stéphane ROCHE via ChimeraX-users wrote:
>
> Dear Mr/Mrs,
>
> I am new to ChimeraX (v 0.7) and I have a trouble when saving a map.
>
> I am working with a .ccp4 map from an electron microscopy project. I 
> would like to slightly change the pixel size that was inaccurately 
> estimated during data processing and chimeraX seems to be the program 
> for doing that.
>
> -Opening the map is fine.
>
> -Then I change the pixel voxelsize which is also fine.
>
> -I then save the modified map (I use the .mrc format here given that I 
> do not find a .ccp4 format).
>
> -However if I then use the map with my usual softwares (phenix, coot) 
> it looks like pure noise. But the map looks normal with some other 
> softwares (e.g. pymol). I also have no problem usually with my .mrc 
> maps with any software so I would assume the problem is with the .mrc 
> map I created
>
> Do you know what I could do to solve this problem ?
>
> Thanks,
>
> Stephane Roche
>
> Institut de Biologie Intégrative de la Cellule
>
> 91198 Gif sur Yvette
>
> France
>
>
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