[chimerax-users] Colab Modelled, but no PDB

Anthony Morgan anthony.morgan at pharm.ox.ac.uk
Sat Jul 23 04:41:05 PDT 2022


Dear All,

I’m new to Chimera X and to Colab Alphafold (AF), hope you can help please.


  *   I’m running Chimera X 1.4 on a Macbook Air.
  *   I want the predicted structure of a fairly large protein (1182 residues).
  *   When I first ran Colabfold_predict, it terminated after 90 min (RAM issue).
  *   I re-ran the same sequence *without * Energy Minimization.
  *   After 20 mins, it successfully returned the first model (see attached), but ran into RAM issues again.

Two questions:


  *   There is no ‘Prediction’ folder in my Downloads, so how can I download the *.pdb file of model #1 that it clearly has generated?
  *   If I cannot do this, will an upgrade to Colab Pro solve this issue for this size of protein?

Thanks for advice.

Kind regards,

Anthony

[cid:8B6C8DAE-A4C3-4F95-8B26-037B6B318679]




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