[chimerax-users] Re-scaling maps

Elaine Meng meng at cgl.ucsf.edu
Thu Jul 28 08:58:11 PDT 2022


Hi Nebojša,
See the ChimeraX "volume" command "voxelSize" option:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/volume.html#dimensions>

The Chimera "volume" command has the same option:
<https://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/volume.html#dimensions>

... and the Chimera Volume Viewer tool (GUI) has the voxel size setting in the Coordinates section (which you would show in the GUI using the Features menu):
<https://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/volumeviewer/volumeviewer.html#features>

I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Chimera(X) team
Department of Pharmaceutical Chemistry
University of California, San Francisco

> On Jul 28, 2022, at 6:38 AM, Nebojsa Bogdanovic via ChimeraX-users <chimerax-users at cgl.ucsf.edu> wrote:
> 
> Hello,
> 
> I have two maps. One from cryosparc @ 1.06 A/pix and another EM-gan sharpened map @ 1.0 A/pix. They are different in size upon visual inspection in chimeraX so I tried relion_image_handler --i cryosparc.mrc --angpix 1.0 --rescale_angpix 1.06 --o cryosparc-rescaled.mrc however, the resulting map is still smaller than the original input. I also tried to rescale in this way to several different angpix values but maps are still not the same.
> 
> Is there any command in chimeraX or chimera to play with pix sizes to find the right one?
> 
> Thank You and regards,
> 
> Nebojša Bogdanović, Ph.D
> Department of Physiology and Biophysics,
> School of Medicine,
> Case Western Reserve University




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