[chimerax-users] ISOLDE and secondary structure restraints

[Guido Hansen]

Hi all, 

I just started model building in cryoEM maps and get great results using
ISOLDE on AlphaFold2-derived initial models. I have a question which is
mainly directed to Tristan I guess: 

Is is possible to use the command line/script to apply secondary
structure restraints in ISOLDE? I would like to restrain the initial
secondary structure assignment from Alphafold especially at resolution
worse than 3.5 A. At the moment I select each and every residue range
assigned to helix, parallel or antiparallel beta-strand one after the
other and turn on the corresponding restrains in the ISOLDE GUI. Is
there a better way via command line of script?

Cheers,
Guido 

-- 

PD Dr. Guido Hansen
Group Leader

 UNIVERSITÄT ZU LÜBECK
 INSTITUT FÜR BIOCHEMIE 

 Tel +49 451 3101 3122
 Fax +49 451 3101 3104
E-Mail hansen at biochem.uni-luebeck.de
www.biochem.uni-luebeck.de [1]

 Ratzeburger Allee 160
 23562 Lübeck 

 

Links:
------
[1] http://www.biochem.uni-luebeck.de
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