[chimerax-users] align 400 copies of the same density map into a circle

SHIQING LIAO shiqing03 at g.ucla.edu
Thu May 12 18:56:46 PDT 2022


Hi there,

I am a new user and would really appreciate any input on the task I want to
do using Chimerax.

Currently I have a mrc map, and it is a part of a cylindrical tube-shaped
structure (like the area I drew in the picture). The map looks like a
curved plane from the side of the cylinder, if it helps. If I piece 400
identical copies of map with some rotation and transition, I would ideally
get a cynlider shaped strutcure, without top and bottom (like the picture
below).

The reason why I want to piece the mrc together is that I don't have the
full look of the tube, but I know the rough radius (that's how 400 was
derived), and I want to make a model for the full tube, using the model of
the composing protein. I managed to manually fit the models of individual
protein into one map. For the other mrc copies, I tried to do the same
again but it took a lot of time and not very accurate, so I'm looking for
any coding solutions that might help.

I'm currently thinking if it is possible for me to place a marker at the
center of one mrc map. Then make 400 copies of the map with the marker.
Then write a code to tell each 1/400 mrc map to rotate 0.9 degrees around
the marked center and move to a coordinate. Then I can repeat the same for
the protein models I mapped to one mrc map. But I have no idea how to
achieve it, and any suggestions would be greatly appreciated! Thank you so
much for helping me!!!

Best,
Shiqing

[image: cf23fde5-632f-4568-9700-9307f24e0f6c.png]
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