[chimerax-users] Bond Rotation Questions

[Eric Pettersen]

Hi Chun Po Fung,
	There's a disulfide bond between residues 535 and 547 in that structure, so there is no way to rotate the backbone bonds of residues 543/544 without breaking a bond somewhere.  To do the rotation you will to delete some bond, possibly that disulfide bond I mentioned (and maybe others -- I didn't do an exhaustive examination of the structure).

--Eric

	Eric Pettersen
	UCSF Computer Graphics Lab


> On Nov 15, 2022, at 10:32 PM, FUNG Chun Po via ChimeraX-users <chimerax-users at cgl.ucsf.edu> wrote:
> 
> Hello 
>  
> I have recently tried to use the bond rotation feature of Chimera X (Windows 10), and I have not been able to get it to work.
>  
> I have tried the Bond rotation button on the toolbar, and the torsion command to no avail.
>  
> The bond rotation button does not seem to do anything even when I click and drag with right click.
>  
> The Torsion command states that my bond “is part of a ring/cycle and cannot be rotated” or “To set torsion, middle two atoms (/A ARG 543 N C) must be bonded;they aren't”
>  
> I want to ask how do I use this function to rotate upon a simple amide bond.
>  
> I am not sure am I just selecting the wrong atoms/bonds or is the function just locked for this PDB.
>  
> The PDB file I am using is 7Y4P and I am trying to rotate about residues 543-544.
>  
> I have also tried to do it in normal Chimera’s rotation command with no results.
>  
> Thank you any advice would be welcome.
>  
> Regards,
> Chun Po Fung
>  
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