[chimerax-users] Help Generating a Volume to use for 2D classification template generation

Joshua Denson joshua.denson at usu.edu
Tue Feb 21 13:14:44 PST 2023


Hi,
I’m trying to take an alpha fold model of my complex, generate a low resolution map of the model using molmap, and then use it for 2D template generation in CryoSparc. I am able to generate the low resolution map just fine, but the resulting map is in a rectangular volume (48x64x51) and I need it to be a cubic volume for CryoSparc to accept it. Ideally it would have dimensions of 64x64x64.
Is there a way to generate a low resolution map in a cell with specific dimensions while maintaining the protein complex dimensions? (eg. I don’t want to simply scale it into a cube)
I am using ChimeraX 1.5.

Best Josh

Joshua M. Denson
PhD Candidate (Johnson Group)
Dept. of Chemistry and Biochemistry
Utah State University

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