[chimerax-users] Help Generating a Volume to use for 2D classification template generation

Tom Goddard goddard at sonic.net
Wed Feb 22 10:34:31 PST 2023


Hi Josh,

  I made a ChimeraX feature request to add a molmap option to allow you to specify the grid size.

	https://www.rbvi.ucsf.edu/trac/ChimeraX/ticket/8542 <https://www.rbvi.ucsf.edu/trac/ChimeraX/ticket/8542#ticket>

May be able to add that in a week or two when I am back from vacation.

	Tom

> On Feb 21, 2023, at 1:14 PM, Joshua Denson via ChimeraX-users <chimerax-users at cgl.ucsf.edu> wrote:
> 
> Hi, 
> I’m trying to take an alpha fold model of my complex, generate a low resolution map of the model using molmap, and then use it for 2D template generation in CryoSparc. I am able to generate the low resolution map just fine, but the resulting map is in a rectangular volume (48x64x51) and I need it to be a cubic volume for CryoSparc to accept it. Ideally it would have dimensions of 64x64x64.
> Is there a way to generate a low resolution map in a cell with specific dimensions while maintaining the protein complex dimensions? (eg. I don’t want to simply scale it into a cube)
> I am using ChimeraX 1.5.
>  
> Best Josh
>  
> Joshua M. Denson
> PhD Candidate (Johnson Group)
> Dept. of Chemistry and Biochemistry
> Utah State University
>  
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