[chimerax-users] Question!
Matthew Wengler (TSL)
Matthew.Wengler at tsl.ac.uk
Sun Feb 26 03:04:58 PST 2023
Hello,
I am a PhD student studying the downstream signalling events of a kinase in a fungal plant pathogen. We have differential phosphopeptide data of the kinase itself over a time course during plant infection (i.e., four residues on this kinase are differentially phosphorylated during stages of infection). I had a vision of showing a movie that incorporated the phosphorylated residues x phosphorylation abundance x time on the kinase’s tertiary structure and was wondering if this could be possible with Chimera X:
* The tertiary structure would be displayed in grey
* The phosphorylated residues would be highlighted in blue
* A movie would be played and the highlighted residues would change colour based on phosphopeptide abundance over a time course (ranging from 0-100% abundance = blue -> red)
I was wondering if this was currently possible.
Alternatively (and maybe this is really the easiest way), because we only have ~6 time points, I could have 6 different stills of the protein with the residues highlighted corresponding to the phosphopeptide abundance values (blue-red 0-100%) and then piece them together in a 6 frame video that will loop…? I think I would prefer the colours to blend slower together dynamically than 6 harsh frames.
Would there be a way to do this in Chimera?
Thank you,
Matt Wengler
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